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transscript green one-step qrt-pcr super mix  (TransGen biotech co)

 
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    TransGen biotech co transscript green one-step qrt-pcr super mix
    Detection of miRNA oligonucleotide efficiency by <t>qRT-PCR</t> assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels
    Transscript Green One Step Qrt Pcr Super Mix, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transscript+green+one-step+qrt-pcr+super+mix/pmc07781659-139-14-18?v=TransGen+biotech+co
    Average 90 stars, based on 1 article reviews
    transscript green one-step qrt-pcr super mix - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication"

    Article Title: gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

    Journal: Virulence

    doi: 10.1080/21505594.2020.1864136

    Detection of miRNA oligonucleotide efficiency by qRT-PCR assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels
    Figure Legend Snippet: Detection of miRNA oligonucleotide efficiency by qRT-PCR assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels

    Techniques Used: Quantitative RT-PCR, Transfection, Expressing

    Gga-miR-1603 and gga-miR-1794 negatively regulate viral L gene expression at both the protein and RNA levels . (a, c) DF-1 cells were co-transfected with pCMV-Flag-La L (a) or pCMV-Flag-ZJ1 L (c) and indicated miRNA oligonucleotides. After 24 h, total protein in the cells was harvested for western blotting. The relative expression of L protein was analyzed using Image J software, and this is shown in (b) for the results of (A) and (d) for (C) . (e, f) DF-1 cells were transfected with indicated miRNA oligonucleotides of gga-miR-1603 (e) and gga-miR-1794 (f) for 24 h and then the cells were inoculated with different NDV isolates (0.1 MOI). Total RNA was extracted for qRT-PCR at 24 hpi. Viral L gene expression was normalized to GAPDH using the 2 −ΔΔCt method. * P < 0.05 and ** P < 0.01
    Figure Legend Snippet: Gga-miR-1603 and gga-miR-1794 negatively regulate viral L gene expression at both the protein and RNA levels . (a, c) DF-1 cells were co-transfected with pCMV-Flag-La L (a) or pCMV-Flag-ZJ1 L (c) and indicated miRNA oligonucleotides. After 24 h, total protein in the cells was harvested for western blotting. The relative expression of L protein was analyzed using Image J software, and this is shown in (b) for the results of (A) and (d) for (C) . (e, f) DF-1 cells were transfected with indicated miRNA oligonucleotides of gga-miR-1603 (e) and gga-miR-1794 (f) for 24 h and then the cells were inoculated with different NDV isolates (0.1 MOI). Total RNA was extracted for qRT-PCR at 24 hpi. Viral L gene expression was normalized to GAPDH using the 2 −ΔΔCt method. * P < 0.05 and ** P < 0.01

    Techniques Used: Expressing, Transfection, Western Blot, Software, Quantitative RT-PCR

    Newcastle disease virus (NDV) infection exerts no effect on gga-miR-1603 and gga-miR-1794 expression in vitro . (a, b) DF-1 cells were inoculated with indicated NDV strains (0.1 MOI). The total miRNAs were extracted from cells collected at indicated time points post-NDV infection. Then, the expression levels of gga-miR-1603 (a) and gga-miR-1794 (b) were detected by qRT-PCR. (C, E, F) Three different avian cells lines, including CEF (c), HD11 (d), and LMH (e), were infected with the La Sota strain (0.1 MOI). The expression level of both miRNAs at different time points was measured using qRT-PCR. The relative expression of miRNAs was calculated by normalizing levels to 5S rRNA expression
    Figure Legend Snippet: Newcastle disease virus (NDV) infection exerts no effect on gga-miR-1603 and gga-miR-1794 expression in vitro . (a, b) DF-1 cells were inoculated with indicated NDV strains (0.1 MOI). The total miRNAs were extracted from cells collected at indicated time points post-NDV infection. Then, the expression levels of gga-miR-1603 (a) and gga-miR-1794 (b) were detected by qRT-PCR. (C, E, F) Three different avian cells lines, including CEF (c), HD11 (d), and LMH (e), were infected with the La Sota strain (0.1 MOI). The expression level of both miRNAs at different time points was measured using qRT-PCR. The relative expression of miRNAs was calculated by normalizing levels to 5S rRNA expression

    Techniques Used: Infection, Expressing, In Vitro, Quantitative RT-PCR



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    transscript green one-step qrt-pcr super mix  (TransGen biotech co)
    90
    TransGen biotech co transscript green one-step qrt-pcr super mix
    Detection of miRNA oligonucleotide efficiency by <t>qRT-PCR</t> assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels
    Transscript Green One Step Qrt Pcr Super Mix, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transscript+green+one-step+qrt-pcr+super+mix/pmc07781659-139-14-18?v=TransGen+biotech+co
    Average 90 stars, based on 1 article reviews
    transscript green one-step qrt-pcr super mix - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Detection of miRNA oligonucleotide efficiency by qRT-PCR assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels

    Journal: Virulence

    Article Title: gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

    doi: 10.1080/21505594.2020.1864136

    Figure Lengend Snippet: Detection of miRNA oligonucleotide efficiency by qRT-PCR assay . The indicated miRNA oligonucleotides (100 nM) were transfected into DF-1 cells for 24 h and untreated DF-1 cells were used as a blank control. The expression level of miRNAs was measured by qRT-PCR and normalized to 5S rRNA levels

    Article Snippet: Viral L gene expression in RNA extractions was measured by qRT-PCR using TransScript Green One-Step qRT-PCR Super Mix (TransGen Biotech, China) on a LightCycler 480 machine (Roche, Switzerland).

    Techniques: Quantitative RT-PCR, Transfection, Expressing

    Gga-miR-1603 and gga-miR-1794 negatively regulate viral L gene expression at both the protein and RNA levels . (a, c) DF-1 cells were co-transfected with pCMV-Flag-La L (a) or pCMV-Flag-ZJ1 L (c) and indicated miRNA oligonucleotides. After 24 h, total protein in the cells was harvested for western blotting. The relative expression of L protein was analyzed using Image J software, and this is shown in (b) for the results of (A) and (d) for (C) . (e, f) DF-1 cells were transfected with indicated miRNA oligonucleotides of gga-miR-1603 (e) and gga-miR-1794 (f) for 24 h and then the cells were inoculated with different NDV isolates (0.1 MOI). Total RNA was extracted for qRT-PCR at 24 hpi. Viral L gene expression was normalized to GAPDH using the 2 −ΔΔCt method. * P < 0.05 and ** P < 0.01

    Journal: Virulence

    Article Title: gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

    doi: 10.1080/21505594.2020.1864136

    Figure Lengend Snippet: Gga-miR-1603 and gga-miR-1794 negatively regulate viral L gene expression at both the protein and RNA levels . (a, c) DF-1 cells were co-transfected with pCMV-Flag-La L (a) or pCMV-Flag-ZJ1 L (c) and indicated miRNA oligonucleotides. After 24 h, total protein in the cells was harvested for western blotting. The relative expression of L protein was analyzed using Image J software, and this is shown in (b) for the results of (A) and (d) for (C) . (e, f) DF-1 cells were transfected with indicated miRNA oligonucleotides of gga-miR-1603 (e) and gga-miR-1794 (f) for 24 h and then the cells were inoculated with different NDV isolates (0.1 MOI). Total RNA was extracted for qRT-PCR at 24 hpi. Viral L gene expression was normalized to GAPDH using the 2 −ΔΔCt method. * P < 0.05 and ** P < 0.01

    Article Snippet: Viral L gene expression in RNA extractions was measured by qRT-PCR using TransScript Green One-Step qRT-PCR Super Mix (TransGen Biotech, China) on a LightCycler 480 machine (Roche, Switzerland).

    Techniques: Expressing, Transfection, Western Blot, Software, Quantitative RT-PCR

    Newcastle disease virus (NDV) infection exerts no effect on gga-miR-1603 and gga-miR-1794 expression in vitro . (a, b) DF-1 cells were inoculated with indicated NDV strains (0.1 MOI). The total miRNAs were extracted from cells collected at indicated time points post-NDV infection. Then, the expression levels of gga-miR-1603 (a) and gga-miR-1794 (b) were detected by qRT-PCR. (C, E, F) Three different avian cells lines, including CEF (c), HD11 (d), and LMH (e), were infected with the La Sota strain (0.1 MOI). The expression level of both miRNAs at different time points was measured using qRT-PCR. The relative expression of miRNAs was calculated by normalizing levels to 5S rRNA expression

    Journal: Virulence

    Article Title: gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

    doi: 10.1080/21505594.2020.1864136

    Figure Lengend Snippet: Newcastle disease virus (NDV) infection exerts no effect on gga-miR-1603 and gga-miR-1794 expression in vitro . (a, b) DF-1 cells were inoculated with indicated NDV strains (0.1 MOI). The total miRNAs were extracted from cells collected at indicated time points post-NDV infection. Then, the expression levels of gga-miR-1603 (a) and gga-miR-1794 (b) were detected by qRT-PCR. (C, E, F) Three different avian cells lines, including CEF (c), HD11 (d), and LMH (e), were infected with the La Sota strain (0.1 MOI). The expression level of both miRNAs at different time points was measured using qRT-PCR. The relative expression of miRNAs was calculated by normalizing levels to 5S rRNA expression

    Article Snippet: Viral L gene expression in RNA extractions was measured by qRT-PCR using TransScript Green One-Step qRT-PCR Super Mix (TransGen Biotech, China) on a LightCycler 480 machine (Roche, Switzerland).

    Techniques: Infection, Expressing, In Vitro, Quantitative RT-PCR